Marinangeli - Micropropagation of ornamental geophytes

Pablo Marinangeli*
Centro de Recursos Naturales Renovables de la Zona Semiárida (CERZOS), Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Departamento de Agronomía, Universidad Nacional del Sur, Bahía Blanca (Argentina)

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Key words: bulb, corm, rhizome, in vitro propagation, bioreactors, Lilium, Tulipa, Gladiolus, Alstroemeria.

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Abstract

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Ornamental geophytes are plants with exploited aesthetic properties that survive the unfavorable season by underground storage organs. Tradi tional propagation methods of geophytes species have low efficiency and may be the way for diseases transmission. In vitro propagation can overcome these problems. The procedures for in vitro propagation of bulb crops may differ slightly from the traditional five stages process of micropropagation, since geophytes have such a unique structure. So, a bulb induction stage is necessary after shoot proliferation, while a rooting stage usually is not needed. Efficient geophytes micropropagation systems are described based on the initiation of in vitro culture from perpetuating underground organs and ending with the differentiation of bulblets, propagules that are naturally rustic and can be transferred directly to the ground without acclimatization. However, bulblets and cormlets regenerated in vitro are dormant and must be subjected to treatments in order to release dormancy. Differentiation of shoots and multiplication by shoot clumps can be achieved in most geophytes by direct organogenesis, indirect organogenesis and somatic embryogenesis. The multiplication system influences both the genetic stability and the multiplication rate of propagules. Usually, direct organogenesis is preferred and usually high proliferation rates are achieved. The use of bioreactors for micropropagation has proved applicable to many species and plant organs including shoots, bulbs, microtubers, corms and somatic embryos. However, transplanting shoots in the soil is a major constraint of bioreactor technology for mass propagation of plants due to the fact that most shoots are etiolated and tender and they are easily damaged by handling or environmental stress. It is not the case of geophytes because the hard storage organs produced in vitro can be handled for direct planting in the field; so, these organs are perfectly suitable for mass propagation by using bioreactors. Bioreactor techniques for large-scale propagat ion of geophytes were described for Li l ium, Gladiolus, Hyacinth, Hippeastrum and Ornithogalum, but only a few have found their way to practical application. The most advanced techniques for the micropropagat ion of Tul ipa, Li l ium, Gladiolus and Alstroemeria are reviewed in this paper.

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